Toughman decoy dolly
WebIn this chapter, we introduce how to design and generate miRNA tough decoy inhibitors with an adeno-associated viral construct. TuD inhibitors will have two miRNA binding sites. The TuD will include stem sequences, a miRNA binding site, and linkers. In vitro validation experiments to confirm the effectiveness of the TuD to inhibit miRNA are ...
Toughman decoy dolly
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WebSep 21, 2024 · However, the sponge or tough decoy would have no phenotypic effect on the normal adult mouse heart, as previously described with miRNA inhibitors (Bernardo et al., 2012b). If significant miR-34 knockdown was achieved, the next goal was to test the miR-34 sponges/tough decoy in HF mouse models in which we would expect significant miR-34 … WebAug 31, 2024 · 2. Pre-warm SOC medium (1 mL for each tube) in a 37 °C water bath and LB agar plate containing 50 μg/mL of kanamycin at 37 °C incubator for 1 h. 3. Pre-cool the microcentrifuge tube and electroporation cuvette on ice for at least 15 min. 4. Thaw BJ5183-AD cells on ice (approximately 10 min) and gently mix cells.
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