WebIn general, THP-1 cells exhibit a large, round, single-cell morphology. The cells were derived from the peripheral blood of a 1-year-old human male with acute monocytic leukemia. Some of their characteristics are: [1] Expression of Fc receptor and C3b receptors while lacking surface and cytoplasmic immunoglobulins. Production of IL-1. WebApr 5, 2024 · First, the concentration of PMA being used is at least 4 times higher than needed. 50ng or 125nM is the recommended conc. which also worked well in my pair of …

The use of THP-1 cells as a model for mimicking the function and ...

WebFix the cells with 4% formaldehyde for 20 minutes at room temperature by adding formaldehyde directly to the culture media and adjust to approximately 1 x 10 6 cells/mL. Add 1 mL of the cell solution to a 1.5 mL microfuge tube. Spin down the cells for 30 seconds in a microfuge. Pour off the supernatant and resuspend the cell pellet in 1 mL of ... WebSep 1, 2024 · To avoid variable results using primary human cells due to the genetic background of donors because of the alterable expression of receptors, we decided to perform the experiments in a cell line cultured in the laboratory, emulating the human monocyte, and we choose the human cell line THP-1 [21].This cell line has been … list of tarokka cards

Authenticated THP-1 Cell Line Sigma Aldrich

WebOct 7, 2024 · THP-1 cells (ATCC® TIB202™) were cultured using RPMI-1640 (Life Technologies) supplemented with 2 mM L-glutamine, 10% FCS and Penstrep (100 U/ml) at 37°C, 5% CO 2.Cells were grown to a density of 5 × 10 5 –1 × 10 6 cells/ml and used for experiments between passage 5 and 12. For THP-1 compound treatment and … WebJul 7, 2016 · M1 or M2 phenotype is stable following co-culture with breast cancer cells in hepatic microphysiologic system. Human THP-1 cells are a widely used model for differentiated tissue macrophages that closely resembles native monocyte-derived macrophage differentiation [].To explore the interaction between macrophages and breast … WebTHP-1 cells were pre-labeled with 2ʹ,7ʹ-bis-(2-carboxyethyl)-5-(and-6)-carboxyl-fluorescein acetoxymethyl ester (BCECF-AM) (Molecular Probes, ... After fixation, HUVECs were washed twice with phosphate buffered saline with tween 20 (PBST) and incubated with blocking buffer (4% BSA in PBST) for 1 hour at room temperature. immigration court address new york