WebJul 18, 2007 · Here we report the crystal structure of an inactive T4 endo VII(N62D) complexed with an immobile four-way junction with alternating arm lengths of 10 and 14 base pairs. The junction is a hybrid of the conventional square-planar and stacked-X conformation. Endo VII protrudes into the junction point from the minor groove side, … WebJan 1, 2001 · Phage T4 endonuclease VII (Endo VII), the first enzyme shown to resolve Holliday junctions, recognizes a broad spectrum of DNA substrates ranging from branched DNAs to single base mismatches. We have determined the crystal structures of the Ca2+-bound wild-type and the inactive N62D mutant enzymes at 2.4 and 2.1 A, respectively.
Elimination of inter-domain interactions increases the cleavage ...
WebApr 15, 2014 · Superposition of the Dra III closed structure to Hpy 99I, Pac I and T4 Endo VII shows the high structural conservation of the HNH active site (Fig. 4 ). Figure 4 Comparison of the sequence and structures of HNH REases. (A) Sequence alignment of the Dra III, Hpy 99I, Pac I, T4 Endo VII and Kpn I HNH catalytic motif. WebMay 27, 2011 · T4 Endo VII Resolvase Cleavage Sites in Y-DNAs. The 90-bp Y-DNA was cleaved by the T4 Endo VII resolvase as seen by mobility shift assay. The cleaved Y … tire and installation near me
F-CphI represents a new homing endonuclease family using the Endo VII ...
WebJul 18, 2007 · Phage T4 endonuclease VII (endo VII) was the first enzyme shown to resolve Holliday junctions into duplex DNAs by introducing symmetrical nicks in equivalent … WebOct 4, 2007 · Phage T4 endonuclease VII (endo VII) was the first enzyme shown to resolve Holliday junctions into duplex DNAs by introducing symmetrical nicks in equivalent … WebJan 30, 2024 · SABATO 30 GENNAIO 2024 (@)jje/\ INN IL PICC OL, 140 DELLE MIGLIORI MARCHE ETEICNA Il Il] DI 771592169468 I GNN VU, MONTATURE DA VISTA ED OCCHIALI DA SOLE TUTTO 01e25,00 QUOTID tire and lube center raf lakenheath