Pnpg assay for beta glucosidase
WebJan 1, 2024 · Abstract. β-Glucosidase (β- d -glucoside glucohydrolase; EC 3.2.1.21) (BGL) is a ubiquitous, well-characterized, biologically important enzyme that catalyzes the transfer of glycosyl groups between oxygen nucleophiles. It plays pivotal roles in many biological processes, and because of its hydrolysis as well as transglycosylation properties ... WebJan 15, 2024 · A simple and portable method for β-Glucosidase activity assay and its inhibitor screening based on a personal glucose meter In this study, a simple and portable …
Pnpg assay for beta glucosidase
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Webglucosidase inhibitory assay. From the results showed the highest activity of Caesalpinia pulcherrima leaves to α-glucosidase inhibition. Thereby this plant is possible a candidate for diabetic treatment. Since both IC50 and proportion (0.32 folds) of this plant were nearly too same values as positive control. However, the slope of linear WebPlace the following reagents in a test tube and equilibrate in the water bath at 37°C for about 15 minutes: Acetate buffer, pH 5.0 1.0 ml PNPG solution 0.5 ml. Add 0.5 ml of the enzyme solution and mix. After the test tubes have incubated for exactly 15 minutes at 37°C, add 2.0 ml of Na2CO3 solution to stop the reaction (ODtest).
WebMay 19, 2024 · The recombinant β-glucosidase was marked by arrows. e pNPG assay on enzymatic activity of wild type and prokaryotic expressed β-glucosidase without signal peptide. BL21-pET28a-ΔSP represents recombinant plasmid without bgl signal peptide. Bars represent mean ± SD ( n = 3). * p < 0.05; ** p < 0.01 Full size image
WebThe beta-glucosidase exhibited low levels of activity against p-nitrophenyl-beta-D-fucopyranoside (13.5%) and p-nitrophenyl-alpha-D-glucopyranoside (7.0%), compared to its activity against p-nitrophenyl-beta-D-glucopyranoside (pNPG, 100%). WebFeb 11, 2010 · Enzyme assays β-glucosidase activity was determined by measuring the amount of glucose and p -nitrophenol released, respectively, from cellobiose and p -nitrophenyl-β-D-glucopyranoside (pNPG) used as substrates.
Webα-Glucosidase Inhibition Assay The α-glucosidase inhibition assay was conducted to evaluate the conversion of PNPG into p-nitrophenol by α-glucosidase and yellow color was measured at 405 nm via spectrophotometer. 35 For this assay, acarbose was used as a reference compound and the reactions were conducted on 96 well plates.
WebConclusion: Our results suggested AMPK/PI3K/Akt signaling is an important way for the anti-type 2 diabetic activity of silkworm excrement by using an integrated approach based on network pharmacology combined with experimental verification. Keywords: α-glucosidase, insulin resistance, network pharmacology, silkworm excrement, type 2 diabetes. homewoodbarbershop.comWebMay 1, 2007 · The enzyme catalyzes the hydrolysis of p -nitrophenyl-β- d -glucopyranoside (PNPG) as the substrate, with a Km of 1.52 mM, and Vmax of 3.21 U min mg −1 protein. Glucose competitively inhibited β-glucosidase with a Ki value of 0.79 mM. Optimal activity with PNPG as the substrate was at pH 5.0 and 50°C. histogram statistical analysisWebassay tested the enzyme with the substrate p-Nitrophenyl β-D- glucopyranoside and 50mM pH 5 sodium acetate buffer. The products of the reaction between this substrate and β-glucosidase are para-nitrophenol and glucose. Para-nitrophenol is yellow in colour under alkaline conditions. The pH assay tested the enzyme with the same substrate, but ... homewood baptist church alexandria laWebIn Abcam's Alpha-Glucosidase Activity Assay Kit (Colorimetric) (ab174093), α-Glucosidase hydrolyzes the Substrate Mix to release the p-nitrophenol that can be measured colorimetrically (OD = 410 nm). This is an easy, quick and high-throughput capable kit that can measure 0.1-10 mU of α-glucosidase activity in a variety of samples. histogram steps per day in rWebReducing sugars were measured by the dinitro salicylic acid (DNS) method [20]. One unit of cellulase activity was defined as the amount of enzyme producing 1 μmol of reducing sugars per minute. Beta-glucosidase (BGL, stated as UBGL) activity determination was performed by the use of 10 mM ρ-nitrophenol-β-glucoside (ρNPG). histogram statisticsWebThe catalytic efficiency of Y-1-B1 for hydrolyzing pNPG was 1355 mM(-1) s(-1)at 50 degrees C and pH 5, significantly higher than that of 16BGL (807 mM(-1) s(-1)). Y-1-B1 also achieved a slightly reduced strength of product inhibition of 1.1 at a glucose concentration of 20 mM, compared with the ratio of 1.3 for 16BGL. ... beta-Glucosidase ... histogram statistics graphWebThe first assay I used pNPG 1mM (150µL) dissolved in sodium acetate buffer 0,1M pH=4,8, and 10µL of enzymatic extract. The mix was then incubated at 50ºC for 10 min. After that, … histogram statistics calculator