Myh11-creert2 rosa
Web1 apr. 2024 · TL;DR: In this paper, the authors employ Myh11-CreERT2 ROSA floxed STOP eYFP Apoe−/− smooth muscle cell (SMC) lineage tracing mice to show that traditional methods for detecting SMCs based on immuno-staining fail to detect > 80% of SMC … Web26 nov. 2024 · Experiments using MYH11 CreER T2-ROSA floxed STOP-eYFP apolipoprotein E (ApoE)-/-, for example, showed that up to 80% of cells traced with immunostaining alone could be misclassified . Although this system is robust, a few concerns with the system still exist. Expression of the targeted genes in visceral SMCs is …
Myh11-creert2 rosa
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Web28 aug. 2024 · The Myh11-CreER T2 mouse line (Cre +) has gained increasing application because of its high lineage specificity relative to other Cre drivers targeting smooth muscle cells (SMCs).This Cre allele, however, was initially inserted into the Y chromosome (X/Y … WebMyh11-CreERT2 (but not Itga8-CreERT2) mice exhibited high levels of CreERT2 protein, tamoxifen-independent activity, and an altered transcriptome. Whereas Myh11-CreERT2-mediated knockout of Srf resulted in a lethal intestinal phenotype, loss of Srf with Itga8 …
Web(A) Immunofluorescence image of the gastric muscle layer in a Myh11 CreERT2 ;Rosa LSL-YFP/+ mouse prior to tamoxifen injection. Gastric section of a 6-week-old mouse stained for YFP (green) and SMA (red). Nuclei were stained with DAPI. Scale bar = 100 … WebThe Jackson Laboratory double heterozygous myh11 creert2 rosa26 il11 Double Heterozygous Myh11 Creert2 Rosa26 Il11, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS …
WebFigure Legend Snippet: (A,B) c-Myc in situ hybridization on ctrl (A) and Myh11-cre;c-Myc f/f (B) lungs 7 days after naphthalene injury, showing that c-Myc is expressed by airway smooth muscle cells after airway epithelial injury and the absence of c-Myc expression in … WebStill need help finding something? Call 1.800.422.6423 (US) 1.207.288.5845 (International) M-F 8am - 8pm est or email us at [email protected]
Web29 jul. 2024 · The final genotypes of the SMC lin Tcf21 knockout (SMC lin-KO) mice were: Tg Myh11-CreERT2, Tcf21 ∆SMC/∆SMC, ROSA tdT/+ and ApoE −/ ...
http://ampk-inhibitor.com/2024/06/05/supplementary-materialssupplementary-information-41467_2024_8811_moesm1_esm-analyses-demonstrate-that-manifestation-of-the-migratory/ scythe\\u0027s leWebThe Myh11-CreERT2 ROSA STOPfl/fl tdTomato reporter mouse labels pericytes in the lung capillary bed. (A) Representative immunofluorescence (IF) micrographs of lung sections stained for tdTomato... peabody campusWebMale Myh11 Creert2, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, ... Article Snippet: Heterozygous Rosa26-IL11 (C57BL/6 N- Gt(ROSA)26Sor tm1(CAG-Il11)Cook /J) mice … peabody care and support servicesWebMouse Resources & Model Organism Links; Phenotypes & Mutants Community Resources; Nucleic Acids Research (Journal) Database Summary; Mouse Facts peabody cafeteriaWeb23 aug. 2024 · Myh11-CreERT2;Tgfbr2 fl/fl transgenic mice were injected with tamoxifen to delete TGF-β receptor ... Laboratory, stock 007576, C57BL background) contain a cell membrane–targeted, 2-color fluorescent Cre-reporter in the ROSA locus. These mice express TdTomato (mT) fluorescent protein in non–Cre recombinase–expressing cells … scythe\\u0027s l3Web10 jan. 2024 · Shankman et al. demonstrated that the contribution of SMCs within atherosclerotic plaques has been extremely neglected and that the transformation of the SMC phenotype mediated by KLF4 is crucial in lesion pathogenesis utilizing a Myh11-CreERT2 ROSA floxed STOP eYFP ApoE −/− mouse model . peabody campus vanderbiltWebThis assay will NOT distinguish hemizygous from homozygous transgenic animals. The genotyping protocol (s) presented here have been optimized for reagents and conditions used by The Jackson Laboratory (JAX). To genotype animals, JAX recommends researchers validate the assay independently upon receipt of animals into their facility. scythe\u0027s l6