Hcd2 cre
WebMay 23, 2016 · Lck Cre-mediated deletion of Cxxc1 led to the similar thymocyte and peripheral T cell developing defects as seen in Cxxc1 fl/fl hCD2 Cre mice (Supplementary Fig. 3a–e), although less severe defects were found in Cxxc1 fl/fl Lck Cre due to the late expression of Cre recombinase and possible leaky deletion of the Cxxc1 gene by Lck … WebApr 16, 2013 · To generate the Aid-cre-cd2 construct, an expression cassette of cre IRES hCD2, the intracellular domain of which was deleted, was inserted into exon 2 of mouse Aicda on the BAC by homologous recombination in bacteria, as described previously [32] ( …
Hcd2 cre
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Web008520 B6.Cg-Tg(CD2-icre)4Kio/J Mice harboring the hCD2-iCre transgene have the human CD2 promoter and locus control region (LCR) directing expression of an … WebOct 12, 2024 · CD4 conv iCreERT2-hCD2: A temporally controlled Cre line for conventional CD4 + T cells. Subpopulations of CD4 + T cells have distinct functions, such as CD4 + …
WebApr 17, 2015 · Vav-iCre expresses active Cre in fetal and adult hematopoietic stem cells and all descendants, hCD2-iCre in immature and mature B and T lymphocytes. To better characterize which hematopoi-etic... WebCommon Name: hCD2-iCre Mice harboring the hCD2-iCre transgene have the human CD2 promoter and locus control region (LCR) directing expression of an optimized variant of Cre recombinase (iCre) to T cells and B cells (all B cell and T cell progenitors) and mice may be useful for generating conditional mutations in T cells and B cells.
WebDec 16, 2016 · The Colorado Department of Public Health and Environment (CDPHE) has conducted statewide laboratory-based surveillance of CRE since November 2012. CRE … WebHere we demonstrate that functional ILC2 cells can arise in the embryonic thymus from shared T cell precursors, preceding the emergence of CD4 + CD8 + (double-positive) T …
WebGeneration of hCD2-cre Transgenic Mice—The plasmid phCD2-cre, placing cre recombinase under the control of hCD2 promoter (21), was microinjected into mouse oocytes to obtain the transgenic mice. Generation of H19exI Mice—We used cre-loxP-based deletion strat-egy for generating the mutants (22). As shown in Fig. 1b, the targeting
WebJun 18, 2024 · In humans, CD2 expression was reported to distinguish pDC subsets 31,32, whereas in hCD2-iCre +/− R26-stop-EYFP +/− reporter mice, in which every cell expressing Cd2 will be irreversibly ... integrity and authenticity in cryptographyWebApr 1, 2012 · hCD2-Cre transgenic Cre expression is lymphocyte specific and present in NK cells at early stages of NK development, and maturing NK cells are YFP positive early in maturation. ( A) Schema of Dicer1 / Rosa26 excision in the absence ( top) or presence ( bottom) of the hCD2-Cre transgene. joe on a boat noahsnoahWebApr 17, 2015 · Two widely used transgenes for conditional gene recombination in hematopoietic cells are Vav-iCre driven from the mu-rine Vav1 promotor, and hCD2-iCre … integrity and authenticity meaningWebThPOKCreERT2-hCD2: A temporally controlled Cre line for CD4+ T cells Tools to selectively target CD4+ T cells and their subpopulations … joe on impractical jokers net worthintegrity and accountability in the workplaceWebDec 28, 2011 · The Aicda coding region on the transgene was engineered to generate the human CD2 and Cre recombinase instead of AID; thus, current Aicda promoter activation can be visualized by hCD2 staining. joe onoufriou scholarWebDec 28, 2024 · Preliminary studies showed that adoptively transferred hCD2-Cre+ Tcf7FL/FL Lef1FL/FL ( Tcf7−/−Lef1−/−) CD4 + T cells homed to secondary lymphoid organs at ∼50% efficiency of wild-type (WT) cells, with similar survival capability ( … integrity and character in the bible