WebMay 28, 2024 · The h-CLAT assay was conducted following OECD TG 442E. NPs inducing relative fluorescence intensity of CD86 ≥ 150% and/or CD54 ≥ 200% in at least two out of three independent runs were predicted to be positive. Thus, Ag (20 nm, 50 nm, and 80 nm) NPs and ZnO NPs were all predicted to be positive in terms of SS possibility using the h … WebNov 3, 2024 · The highest predictivity was obtained for the approach in which sensitization potential was determined by Spectro-DPRA followed by final determination using the result of KeratinoSens™ and h-CLAT assays (96.3% sensitivity, 87.1% specificity, 86.7% positive predictive value, 96.4% negative predictive value and 91.4% accuracy compared with ...
Implementation of Three in-vitro Test Methods for …
WebAug 1, 2024 · The human cell line activation test (h-CLAT) is an OECD approved (Test No. 442E) assay to identify novel skin sensitizers. h-CLAT simulates dendritic cell activation … WebHome - National Toxicology Program damage amp script
Human Cell Line Activation Test (h-CLAT) - Eurofins Deutschland
WebHuman Cell Line Activation Test (h-CLAT) In Vitro Skin Sensitisation Assay The in vitro h-CLAT addresses the third molecular key event of the adverse outcome pathway (AOP) … WebApr 16, 2024 · One of the animal-free alternative assays accepted by OECD testing guidelines is the human cell line activation test (h-CLAT) 8. The h-CLAT measures in vitro DC activation by selective induction of the surface markers CD54 and CD86 in human THP-1 monocytic cell line 9-11. This method evaluates the variation in the expression of these … WebLuSens, h-CLAT, U-SENSTM or IL-8 Luc Assay – should be used alone to fulfil REACH information requirements. They should always be considered in combinations and/or with other information. However, the kDPRA assay can be used as a standalone assay if the result indicates that Sub-category 1A would need to be applied. More information on the ... marin immigration law