Gene-indexed mutations in maize
WebMar 5, 2024 · Gene-Indexed Mutations in Maize Introduction. Maize is an important staple crop, often used as feed and processed as a raw material in the food... Results. Given … WebGene-indexed mutations in maize (Zea mays) A collection of genome-wide mutations in maize is generated through ethyl methanesulfonatemutagenesis. The mutations are detected and located by combining exome capture and next-generation sequencing. A rapid and low-cost pipeline for gene identification is developed.
Gene-indexed mutations in maize
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WebThe development of site-directed mutagenesis and sequenced-indexed genetics resources in maize allows for targeted analysis of individual genes. These reverse genetics approaches have the potential for confirmation bias by only studying candidate genes for association with traits of interest. WebForward genetics in maize has been very fruitful and there are several mutant populations in maize; however, most of these are in standard inbred backgrounds. Most induced mutants in maize were created by two methods: pollen mutagenesis with Ethyl Methanesulfonate (EMS) or crossing with maize lines that carry highly active …
Webgenetics resources for characterizing gene knockouts in maize. Results A sample of 106 sequence-indexed insertion sites from the UniformMu population were tested for … WebDec 7, 2024 · Search life-sciences literature (41,931,802 articles, preprints and more) Search. Advanced search
WebWe developed a novel Ethyl Methanesulfonate (EMS) induced mutation resource in maize comprising 2050 independent M2 mutant families in the elite tropical maize inbred ML10. … WebGene-Indexed Mutations in Maize. Mol Plant. 2024; 11(3):496-504 (ISSN: 1752-9867) ... We have generated a gene-indexed maize mutant collection through ethyl methanesulfonate mutagenesis and detected the mutations by combining exome capture and next-generation sequencing. A total of 1086 mutated M1 plants were sequenced, …
WebMar 6, 2024 · Mapping-by-Sequencing via MutMap Identifies a Mutation in ZmCLE7 Underlying Fasciation in a Newly Developed EMS Mutant Population in an Elite Tropical Maize Inbred. Quan Hong Tran Department of Genetic Engineering, The Agricultural Genetics Institute, Km2 Pham Van Dong Street, Hanoi, Vietnam. Search articles by …
WebA subset of these putative mutants was tested for genetic heritability. Second, we sequenced 430,000 transposon flanking sequence tags to identify germinal insertions in 144 defective kernel mutations. Third, we identified the maize U2AF35 related gene, ZmURP, as the most likely gene that is responsible for the rgh3 mutant phenotype. thor new movie 2021WebOct 21, 2024 · Here, we generated a new sequence-indexed maize Mutator insertional library named ChinaMu through high-throughput sequencing of enriched Mu -tagged sequences. A total of 2,581 Mu F2 lines were analyzed, and 311,924 nonredundant Mu insertion sites were obtained. thorne witches seriesWebDec 1, 2024 · The genome-wide mutation collection of gene indexes obtained by using B73 pollen with EMS treatment provides an essential resource for the functional analysis of … umur pemakaian print printhead dot matrixWebThe availability of the B73 inbred reference genome sets the stage for high-throughput functional characterization of maize genes on a whole-genome scale. Among the 39 324 … thor new movie downloadWebMay 9, 2007 · In maize, FSTs have been generated using DNA transposons. Transposable elements can generate unstable insertions that are difficult to analyze for simple … thor new movie castWebMaize needs to strive for the benchmark established in A. thaliana, in which sequence-indexed insertional mutations can be ordered for almost every gene. These resources are now being developed ... umusic broadwaterWebMay 9, 2007 · In maize, FSTs have been generated using DNA transposons. Transposable elements can generate unstable insertions that are difficult to analyze for simple knockout phenotypes. Transposons can also generate somatic insertions that fail to segregate in subsequent generations. Results umusic customer care phone number